Polymerase chain reaction with lesion scrapping for the diagnosis of human American tegumentary leishmaniasis
Mem. Inst. Oswaldo Cruz
;
101(4): 427-430, June 2006. ilus, tab
Article
in English
| LILACS
| ID: lil-435305
ABSTRACT
The objective of this work was to compare the polymerase chain reaction (PCR) using lesion scrapping with other conventional techniques for the diagnosis of the American tegumentary leishmaniasis (ATL). For this, patients with cutaneous lesions suspected to be ATL were studied. The DNA was amplified with the MP1L/MP3H primers. From the 156 studied patients, 79 (50.6 percent) presented positive parasite direct search (PD), 81 (51.9 percent) had positive Montenegro skin test (MST), and 90 (57.7 percent) presented PD and/or MST positive. The PCR was positive in all of the positive-PD patients (100 percent sensitivity), in 91.1 percent of the positive PD and/or MST patients, and in 27.3 percent of the patients that presented negative PD and positive MST. The PCR positivity was similar to the PD (P = 0.2482) and inferior to the MST (P = 0.0455), and to the PD/MST association (P = 0.0133). The high PCR sensitivity, and positivity in those cases where the PD was negative, highlights the importance of this technique as an auxiliary tool for the diagnosis of ATL.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Polymerase Chain Reaction
/
DNA, Protozoan
/
Leishmaniasis, Cutaneous
/
Leishmania
Type of study:
Diagnostic study
Limits:
Aged80
/
Animals
/
Female
/
Humans
/
Male
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2006
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Estadual de Maringá/BR
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