Isolation of respiratory syncytial virus from nasopharyngeal aspirates stored at 20°C from one to fifteen months after collection
Mem. Inst. Oswaldo Cruz
;
101(4): 451-453, June 2006. tab
Article
in English
| LILACS
| ID: lil-435308
ABSTRACT
Cell culture isolation is used for recovering respiratory syncytial virus (RSV) from respiratory specimens. As RSV is a thermolabile virus, specimens destined for inoculation into cell culture require special transport, handling, and storage. The isolation rate of RSV from nasopharyngeal aspirates (NPA) stored at 20°C for one to 15 months after collection was investigated. A total of 126 samples considered positive for RSV by indirect fluorescence-antibody were tested by virus isolation in HEp-2 cell culture. RSV was isolated from 47/126 specimens (37.3 percent). These results show that RSV may be recovered from NPA stored at 20°C by cell culture.
Full text:
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Index:
LILACS (Americas)
Main subject:
Respiratory Syncytial Viruses
/
Nasopharynx
/
Respiratory Syncytial Virus Infections
Limits:
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2006
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Federal do Ceará/BR
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