Molecular characterization of human Trypanosoma cruzi isolates from endemic areas in Panama
Mem. Inst. Oswaldo Cruz
;
101(4): 455-457, June 2006. ilus, tab
Article
in English
| LILACS
| ID: lil-435309
ABSTRACT
The present work provides information on Trypanosoma cruzi genotype circulating in endemic areas of Chagas disease in Panama. A total of 26 crude stocks of T. cruzi, isolated from the blood of persons with different clinical profiles of Chagas disease were collected and crio-conserved until used. Most of the stocks had been characterized by means of isoenzyme electrophoresis on cellulose acetate membranes. The clinical profiles of infected persons included 9 (34.6 percent) asymptomatic and 17 acute (65.4 percent) including 5 (19.2 percent) fatal cases, 2 under 5 years old and 3 adults. A multiplex-PCR assay based on the amplification of the non-transcribed spacer of the mini-exon gene was performed. All stocks of T. cruzi included in the study were found to correspond to Tc I group. This result supports the predominance of T. cruzi-I in the transmission cycles affecting the human population in the Republic of Panama.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Trypanosoma cruzi
/
Chagas Disease
/
Endemic Diseases
Limits:
Animals
/
Humans
Country/Region as subject:
Central America
/
Panama
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2006
Type:
Article
/
Project document
Affiliation country:
Panama
Institution/Affiliation country:
Instituto Conmemorativo Gorgas de Estudios de la Salud/PA
/
University of Panama/PA
Similar
MEDLINE
...
LILACS
LIS