Comparison between indirect enzyme-linked immunosorbent assays for Anaplasma marginale antibodies with recombinant major surface protein 5 and initial body antigens
Mem. Inst. Oswaldo Cruz
;
101(5): 511-516, Aug. 2006. ilus, graf, tab
Article
in English
| LILACS
| ID: lil-437031
ABSTRACT
Indirect enzyme-linked immunosorbent assays (ELISAs) based on recombinant major surface protein 5 (rMSP5) and initial body (IB) antigens from a Brazilian isolate of Anaplasma marginale were developed to detect antibodies against this rickettsia in cattle. Both tests showed the same sensitivity (98.2 percent) and specificities (100 percent for rMSP5 and 93.8 percent for IB ELISA) which did not differ statistically. No cross-reactions were detected with Babesia bigemina antibodies, but 5 (rMSP5 ELISA) to 15 percent (IB ELISA) of cross-reactions were detected with B. bovis antibodies. However, such difference was not statistically significant. Prevalences of seropositive crossbred beef cattle raised extensively in Miranda county, state of Mato Grosso do Sul, Brazil, were 78.1 percent by rMSP5 ELISA and 79.7 percent by IB ELISA. In the analysis of sera from dairy calves naturally-infected with A. marginale, the dynamics of antibody production was very similar between both tests, with maternal antibodies reaching the lowest levels at 15-30 days, followed by an increase in the mean optical densities in both ELISAs, suggesting the development of active immunity against A. marginale. Results showed that all calves were seropositive by one-year old, characterizing a situation of enzootic stability. The similar performances of the ELISAs suggest that both tests can be used in epidemiological surveys for detection of antibodies to A. marginale in cattle.
Full text:
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Index:
LILACS (Americas)
Main subject:
Bacterial Outer Membrane Proteins
/
Enzyme-Linked Immunosorbent Assay
/
Anaplasma marginale
/
Anaplasmosis
/
Antigens, Bacterial
Type of study:
Diagnostic study
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2006
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Embrapa Gado de Corte/BR
/
Instituto de Biologia Molecular do Paraná/BR
/
Universidade Federal da Bahia/BR
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