Genotipificación de aislados clínicos de Helicobacter pylori en base a genes asociados a virulencia cagA, vacA y babA2: Primer aislamiento de una cepa babA2 positiva en pacientes chilenos / Genotypying of clinical isolates of Helicobacter pylori by cagA, vacA and babA2 virulence associated genes: First detection of a babA2 positive strain in Chilean patients
Rev. méd. Chile
;
134(8): 981-988, ago. 2006. ilus, tab, graf
Article
in Spanish, English
| LILACS
| ID: lil-438368
ABSTRACT
Background:
Helicobacter pylori-associated gastroduodenal diseases depends on host characteristics, environmental conditions and bacterial virulence factors, such as cagA, vacA y babA2 gene products. Moreover, peptic ulcer disease has been related with cagA+, vacAs1m1 strains, while metaplasia and gastric cancer has been associated to cagA+, vacAs1 and babA2+ H pylori strains. Gene babA2 has not yet been described in clinical isolates from Chilean patients.Aim:
To investigate the presence of cagA, vacA (s and m) and babA2 genes in clinical isolates of H pylori from Chilean patients. Material andMethods:
Sixty six isolates from 41 patients were genotyped by PCR, using primers for s1a, s1b, s2, m1, m2, cagA and babA2 genes as previously described.Results:
cagA gene was detected in 16 isolates (24.2 percent) while vacAs1a, vacAs1b, vacAs2, vacAm1 and vacAm2 were detected in 28 (42.4 percent), 14 (21.2 percent), 17 (25.8 percent), 21 (31.8 percent) and 29 isolates (43.9 percent), respectively. One isolate (1.5 percent) was babA2 positive, being the first isolate with this genotype described in Chile. Besides the babA2+ genotype this clinical isolate also presented cagA+ and vacAs1a which has been related with metaplasia or gastric cancer. Five isolates showed an ulcerogenic profile cagA+, vacAs1m1.Conclusions:
The results presented indicate the prevalence of vacAs1m1 genotype among the clinical isolates analyzed, and a low frequency of babA2 genotype.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Bacterial Proteins
/
Helicobacter pylori
/
Helicobacter Infections
/
Adhesins, Bacterial
/
Gastrointestinal Diseases
/
Antigens, Bacterial
Type of study:
Diagnostic study
/
Risk factors
Limits:
Humans
Country/Region as subject:
South America
/
Chile
Language:
English
/
Spanish
Journal:
Rev. méd. Chile
Journal subject:
Medicine
Year:
2006
Type:
Article
/
Project document
Affiliation country:
Chile
/
United States
Institution/Affiliation country:
Ochsner Clinic Foundation/US
/
Universidad de Concepción/CL
Similar
MEDLINE
...
LILACS
LIS