Germline transformation of Aedes fluviatilis (Diptera: Culicidae) with the piggyBac transposable element
Mem. Inst. Oswaldo Cruz
;
101(7): 755-757, Nov. 2006. ilus, tab
Article
in English
| LILACS
| ID: lil-439459
ABSTRACT
The technique to generate transgenic mosquitoes requires adaptation for each target species because of aspects related to species biology, sensitivity to manipulation and rearing conditions. Here we tested different parameters on the microinjection procedure in order to obtain a transgenic Neotropical mosquito species. By using a transposon-based strategy we were able to successfully transform Aedes fluviatilis (Lutz), which can be used as an avian malaria model. These results demonstrate the usefulness of the piggyBac transposable element as a transformation vector for Neotropical mosquito species and opens up new research frontiers for South American mosquito vectors.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Transformation, Genetic
/
DNA Transposable Elements
/
Gene Transfer Techniques
/
Aedes
/
Insect Vectors
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2006
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Fundacao Oswaldo Cruz/BR
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