The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys
Mem. Inst. Oswaldo Cruz
;
101(7): 801-807, Nov. 2006. ilus, graf, tab
Article
in English
| LILACS
| ID: lil-439467
ABSTRACT
There are data indicating that the distribution of Trypanosoma vivax in the Brazilian territory is expanding with potential to reach other areas, where the vectors are present. The detection of anti-trypanosomal antibodies in serum provides important information of the trypanosomal status in cattle herds. For this reason, an enzyme-linked immunosorbent assay (Tv-ELISA-Ab) with crude antigen from one Brazilian isolate of T. vivax was developed and evaluated. The sensitivity and specificity were respectively 97.6 and 96.9 percent. In the evaluation of cross-reactions, three calves inoculated with T. evansi trypimastigotes blood forms showed optical densities (OD) under the cut-off during the whole experimental period, except one at 45 days post-inoculation. With relation to Babesia bovis, B. bigemina, and Anaplasma marginale, which are endemic hemoparasites in the studied area, the cross-reactions were shown to be 5.7, 5.3, and 1.1 percent, respectively. The first serological survey of Pantanal and state of Pará showed that T. vivax is widespread, although regions within both areas had significantly different prevalences. Therefore, this Tv-ELISA-Ab may be a more appropriate test for epidemiological studies in developing countries because the diagnostic laboratories in most countries may be able to perform an ELISA, which is not true for polymerase chain reaction.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Trypanosomiasis, Bovine
/
Antibodies, Protozoan
/
Trypanosoma vivax
/
Antigens, Protozoan
Type of study:
Diagnostic study
/
Prevalence study
/
Prognostic study
/
Risk factors
Limits:
Animals
/
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2006
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Embrapa Gado de Corte/BR
/
Universidade Federal do Pará/BR
/
Universidade para o Desenvolvimento do Estado e da Regiao do Pantanal/BR
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