Identification of a genetic marker associated with the resistance to Schistosoma mansoni infection using random amplified polymorphic DNA analysis
Mem. Inst. Oswaldo Cruz
;
101(8): 863-868, Dec. 2006. graf, ilus
Article
in English
| LILACS
| ID: lil-440573
ABSTRACT
In schistosomiasis, the host/parasite interaction remains not completely understood. Many questions related to the susceptibility of snails to infection by respective trematode still remain unanswered. The control of schistosomiasis requires a good understanding of the host/parasite association. In this work, the susceptibility/resistance to Schistosoma mansoni infection within Biomphalaria alexandrina snails were studied starting one month post infection and continuing thereafter weekly up to 10 weeks after miracidia exposure. Genetic variations between susceptible and resistant strains to Schistosoma infection within B. alexandrina snails using random amplified polymorphic DNA analysis technique were also carried out. The results showed that 39.8 percent of the examined field snails were resistant, while 60.2 percent of these snails showed high infection rates.In the resistant genotype snails, OPA-02 primer produced a major low molecular weight marker 430 bp. Among the two snail strains there were interpopulational variations, while the individual specimens from the same snail strain, either susceptible or resistant, record semi-identical genetic bands. Also, the resistant character was ascendant in contrast to a decline in the susceptibility of snails from one generation to the next.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Schistosoma mansoni
/
Genetic Variation
/
Biomphalaria
/
Random Amplified Polymorphic DNA Technique
Type of study:
Diagnostic study
/
Risk factors
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2006
Type:
Article
Affiliation country:
Egypt
Institution/Affiliation country:
Cairo University/EG
/
National Research Centre/EG
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