Usefulness of argyrophilic nucleolar organizer regions in detection of lung cells alterations after benzo[a]pyrene instillation
Acta cir. bras
;
21(supl.4): 36-39, 2006. ilus, graf
Article
in English
| LILACS
| ID: lil-440777
ABSTRACT
PURPOSE:
To verify the relationship between AgNOR expression and lung tissues changes of Wistar rats after pulmonary instillation of benzo[a]pyrene (B[a]P).METHODS:
Male Rattus norvegicus albinus,Wistar lineage were given a single intrapulmonary instillation of B[a]P at doses of 10 and 20 mg/kg in a volume of approximately 0,3 ml. After 7 and 21 days the rats were killed and the lung slices submitted to a histological technique of AgNOR. AgNOR dots were quantified and the result analyzed by statistical tests; p <= 0,05 was considered significant.RESULTS:
The mean values of AgNOR dots for the experimental groups 10/7 (1,51±0,86) and 10/21 (1,84±0,13) were statistically different (p = 0,009). Among the groups 20/7 (1,63±0,11) and 20/21 (2,48±0,28) was observed statistically significant difference (p = 0,003).CONCLUSION:
The AgNOR technique can be useful in identification of cells changes induced by B[a]P.RESUMO
OBJETIVO:
Verificar a relação entre a expressão de AgNOR e alterações teciduais pulmonares em ratos Wistar após instilação pulmonar de benzo[a]pireno (B[a]P).MÉTODOS:
Rattus norvegicus albinus, linhagem Wistar machos foram submetidos à instilação pulmonar única de B[a]P em doses de 10 e 20mg/kg, em um volume aproximado de 0,3 ml. Os animais foram sacrificados após 7 e 21 dias e o tecido pulmonar submetido a técnica histológica de AgNOR. Os pontos AgNOR foram quantificados e os resultados analisados estatisticamente; foram considerados significantes os valores de p <= 0,05.RESULTADOS:
Os valores médios de pontos AgNOR no grupo experimental 10/7 (1,51±0,86) e 10/21 (1,84±0,13) foram estatisticamente significantes (p = 0,009). Entre os grupos 20/7 (1,63±0,11) e 20/21 (2,48±0,28) a diferença observada foi também considerada significante (p = 0,003).CONCLUSÃO:
A técnica de AgNOR pode ser útil na identificação de alterações celulares induzidas pelo B[a]P.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Nuclear Proteins
/
Carcinoma, Squamous Cell
/
Antigens, Nuclear
/
Lung Neoplasms
/
Nucleolus Organizer Region
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Animals
Language:
English
Journal:
Acta cir. bras
Journal subject:
General Surgery
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2006
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
UFMS/BR
/
UNIDERP/BR
/
UnB/BR
/
University for Development of the State and the Pantanal Region/BR
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