Identification of non-specific alkaline phosphatases in hyphal cells of the fungus Neurospora crassa by in situ histochemistry
Genet. mol. res. (Online)
;
5(3): 483-486, 2006. ilus
Article
in English
| LILACS
| ID: lil-441043
ABSTRACT
The present study was designed to identify alkaline phosphatases in non-permeabilized hyphal cells of the fungus Neurospora crassa by staining these enzymatic activities with a modified azo dye coupling method. Our strategy allowed the identification of three non-specific alkaline phosphatase activities, one of them possibly being a novel putative enzyme, which is not responsive to either Mg2+ or EDTA. Another alkaline phosphatase activity, whose location in the hyphal cell is regulated by phosphate, is stimulated by Mg2+, inhibited by EDTA, and somehow dependent on the expression of the pho-2+-encoded Pi-repressible alkaline phosphatase.
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Index:
LILACS (Americas)
Main subject:
Hyphae
/
Alkaline Phosphatase
/
Neurospora crassa
Type of study:
Diagnostic study
/
Prognostic study
Language:
English
Journal:
Genet. mol. res. (Online)
Journal subject:
Molecular Biology
/
Genetics
Year:
2006
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
USP/BR
/
Universidade de Uberaba/BR
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