Detection of genetic polymorphism among and within Echinococcus granulosus strains by heteroduplex analysis of a microsatellite from the U1 snRNA genes
Genet. mol. res. (Online)
;
5(3): 542-552, 2006. ilus, tab, graf
Article
in English
| LILACS
| ID: lil-441050
ABSTRACT
Polymerase chain reaction of a pentanucleotide microsatellite in the U1 snRNA gene complex generated a multiple band pattern due to the priming of paralogous sequences. Denaturation and slow renaturation of polymerase chain reaction products allow the formation of heteroduplex DNA that can be detected by its differential mobility in polyacrylamide gel electrophoresis. Heteroduplex analysis was used to determine if the U1 snRNA microsatellite could be a useful genetic marker in Echinococcus granulosus. A U1 snRNA microsatellite fragment from E. granulosus was isolated and characterized by Southern blot and sequencing. Four E. granulosus strains were analyzed sheep, Tasmanian sheep, cattle, and camel strains. The former two showed polymorphism and shared three of the six patterns found for sheep strain. The cattle strain displayed two patterns, and the camel strain was monomorphic. The electrophoretic profiles were used for statistical analysis in order to determine genetic distance and the relationship among strains. Heteroduplex analysis can be helpful in genotyping E. granulosus strains and is useful in detecting polymorphism within strains.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Polymorphism, Genetic
/
Ribonucleoprotein, U1 Small Nuclear
/
Microsatellite Repeats
/
Echinococcus granulosus
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
Language:
English
Journal:
Genet. mol. res. (Online)
Journal subject:
Molecular Biology
/
Genetics
Year:
2006
Type:
Article
Affiliation country:
Argentina
/
Brazil
Institution/Affiliation country:
ANLIS Dr. Carlos G. Malbrán/AR
/
Universidade Federal de Santa Maria/BR
/
Universidade Federal do Rio Grande do Sul/BR
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