Aluminum hydroxide associated to Schistosoma mansoni 22.6 kDa protein abrogates partial protection against experimental infection but not alter interleukin-10 production
Mem. Inst. Oswaldo Cruz
;
101(supl.1): 365-368, Oct. 2006. tab, graf
Article
in English
| LILACS
| ID: lil-441276
ABSTRACT
The need to develop a vaccine against schistosomiasis led several researches and our group to investigate proteins from Schistosoma mansoni as vaccine candidates. Sm22.6 is a protein from S. mansoni that shows high identity with Sj22.6 and Sh22.6 (79 and 91 percent, respectively). These proteins are associated with high levels of IgE and protection to reinfection. Previously, we have shown that Sm22.6 induced a partial protection of 34.5 percent when used together with Freund's adjuvant and produced a Th0 type of immune response with interferon-g and interleukin-4. In this work, mice were immunized with Sm22.6 alone or with aluminum hydroxide adjuvant and high levels of IgG, IgG1, and IgG2a were measured. Unfortunately, no protection was detected. Since IL-10 is a modulating cytokine in schistosomiasis, we also observed a high level of this molecule in splenocytes of vaccinated mice. In conclusion, we did not observe the adjuvant effect of aluminum hydroxide associated with rSm22.6 in protective immunity.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Schistosoma mansoni
/
Schistosomiasis mansoni
/
Helminth Proteins
/
Aluminum Hydroxide
Type of study:
Risk factors
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2006
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Hospital Universitário Professor Edgard Santos/BR
/
Universidade Federal de Minas Gerais/BR
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