Candida dubliniensis in a Brazilian family with an HIV 1- infected child: identification, antifungal susceptibility, drug accumulation and sterol composition
Braz. j. microbiol
;
37(3): 237-243, July-Sept. 2006. ilus, tab
Article
in English
| LILACS
| ID: lil-442124
ABSTRACT
This study investigated the prevalence of C. dubliniensis in a Brazilian family with an HIV - infected child. A total of 42 oral isolates were obtained from eight family members. The identification of C. dubliniensis was performed by polymerase chain reactions (PCR) using primers against a specific sequence of the C. dubliniensis cytochrome b gene. Only the HIV-infected child and his grandmother were colonized by C. dubliniensis. In this study C. dubliniensis isolated from the HIV-infected child exhibited high susceptibility for azoles tested with MICs of 0.125 and 0.5 æg/mL for voriconazole and fluconazole, respectively. Accumulation of [³H] fluconazole in C. dubliniensis isolated from the HIV-infected child was slightly reduced in comparison to the reference susceptible strain. C. dubliniensis isolates had significantly lower ergosterol levels in comparison to C. albicans reference strains.
RESUMO
O presente estudo investigou a prevalência de C. dubliniensis em uma família brasileira com uma criança infectada pelo vírus HIV. Um total de 42 isolados orais foram obtidos de 8 membros da família. A identificação de C. dubliniensis foi realizada por polymerase chain reactions (PCR) usando primers contra a sequência específica para o gene C. dubliniensis cytochrome b. Apenas a criança infectada pelo vírus HIV e a avó estavam colonizados por C. dubliniensis. Neste estudo C. dubliniensis isolado da criança infectada pelo vírus HIV exibiu alta susceptibilidade para azoles com concentração mínima inibitória de 0.125 and 0.5 æg/mL para voriconazole and fluconazole respectivamente. Acúmulo de [³H] fluconazol intra-celular foi ligeiramente reduzido em C. dubliniensis isolado da criança infectada pelo vírus HIV em comparação com a cepa referência sensível ao fluconazole. Isolados de C. dubliniensis neste estudo apresentaram níveis significantemente reduzidos de ergosterol da membrane celular em comparação com C. albicans.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
In Vitro Techniques
/
Candida
/
Fluconazole
/
HIV Infections
/
HIV
/
Anti-HIV Agents
/
Ergosterol
Type of study:
Diagnostic study
/
Prevalence study
/
Risk factors
Limits:
Adult
/
Child
/
Female
/
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Braz. j. microbiol
Journal subject:
Microbiology
Year:
2006
Type:
Article
Affiliation country:
Brazil
/
Japan
/
United kingdom
Institution/Affiliation country:
Chiba University/JP
/
Sheffield Children's Hospital/GB
/
Universidade Estadual de Campinas/BR
/
University of Wales Swansea/GB
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