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Production of cellulase-free xylanase by Trichoderma reesei SAF3
Kar, Sanjay; Mandal, Asish; Mohapatra, Pradeep K. Das; Mondal, Keshab C; Pati, Bikash R.
Affiliation
  • Kar, Sanjay; Vidyasagar University. Department of Microbiology. Midnapore. IN
  • Mandal, Asish; Vidyasagar University. Department of Microbiology. Midnapore. IN
  • Mohapatra, Pradeep K. Das; Vidyasagar University. Department of Microbiology. Midnapore. IN
  • Mondal, Keshab C; Vidyasagar University. Department of Microbiology. Midnapore. IN
  • Pati, Bikash R; Vidyasagar University. Department of Microbiology. Midnapore. IN
Braz. j. microbiol ; Braz. j. microbiol;37(4): 462-464, Oct.-Dec. 2006. graf, ilus
Article in En | LILACS | ID: lil-442195
Responsible library: BR32.1
ABSTRACT
A xylanase producing fungi has been isolated from soil and identified as Trichoderma reesei SAF3. Maximum growth of the organism was found at 48 h under submerged condition in xylan containing enriched medium, whereas highest enzyme production (4.75U/mL) was recorded at 72 h. No detectable cellulase activity was noted during whole cultivation period. The partially purified enzyme hydrolyzed xylan into xylopentose and xylose. All these properties of xylanase highlighten its promising uses in industrial scale.
RESUMO
A partir de solo, isolou-se uma cepa de fungos produtos de xilanase, posteriormente identificado como Trichoderma reesei SAF3. O crescimento máximo do fungo foi obtido após 48h em condições submersas em meio de cultura contendo xilano, enquanto produção máxima de enzima (4,75U/mL) ocorreu em 72h. Durante o período de cultivo, não foi detectada atividade celulásica. A enzima parcialmente purificada hidrolizou xilano a xilopentose e xilose. Essas propriedades da xilanase destacam seu uso promissor em escala industrial.
Subject(s)
Full text: 1 Index: LILACS Main subject: Trichoderma / Xylans / In Vitro Techniques / Fungi Language: En Journal: Braz. j. microbiol Journal subject: MICROBIOLOGIA Year: 2006 Type: Article
Full text: 1 Index: LILACS Main subject: Trichoderma / Xylans / In Vitro Techniques / Fungi Language: En Journal: Braz. j. microbiol Journal subject: MICROBIOLOGIA Year: 2006 Type: Article