Identificación de asociaciones clínico-patológicas e hipermetilación de genes supresores de tumores en cáncer gástrico difuso a través de análisis de Hierarchical clustering / Hierarchical clustering analysis to detect associations between clinical and pathological features of gastric tumors and hypermethylation of suppressor genes
Rev. méd. Chile
;
135(1): 17-25, ene. 2007. ilus, tab, graf
Article
in Spanish
| LILACS
| ID: lil-442997
ABSTRACT
Background:
Methylation is an inactivation mechanism for tumor suppressor genes, that can have important clinical implications.Aim:
To analyze the methylation status of 11 tumor suppressor genes in pathological samples of diffuse gastric cancer. Material andmethods:
Eighty three patients with diffuse gastric cancer with information about survival and infection with Epstein Barr virus, were studied. DNA was extracted from pathological slides and the methylation status of genes p14, p15, p16, APC, p73, FHIT, E-caderin, SEMA3B, BRCA-1, MINT-2 y MGMT, was studied using sodium bisulphite modification and polymerase chain reaction. Results were grouped according to the methylation index or Hierarchical clustering (TIGR MultiExperiment Viewer).Results:
Three genes had a high frequency of methylation (FHIT, BRCA1, APC), four had an intermediate frequency (p15, MGMT, p14, MINT2) and four had a low frequency (p16, p73, E-cadherin, SEMA3B). The methylation index had no association with clinical or pathological features of tumors or patients survival. Hierarchical clustering generated two clusters. One grouped clinical and pathological features with FHIT, BRCA1, and APC and the other grouped the other eight genes and Epstein Barr virus infection. Two significant associations were found, between APC and survival and p16/p14 and Epstein Barr virus infection.Conclusions:
Hierarchical clustering is a tool that identifies associations between clinical and pathological features of tumors and methylation of tumor suppressor genes.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Stomach Neoplasms
/
Carcinoma
/
Genes, Tumor Suppressor
/
Promoter Regions, Genetic
/
DNA Methylation
Type of study:
Diagnostic study
/
Risk factors
Limits:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
Language:
Spanish
Journal:
Rev. méd. Chile
Journal subject:
Medicine
Year:
2007
Type:
Article
/
Project document
Affiliation country:
Chile
Institution/Affiliation country:
Hospital Clínico-San Borja Arriarán/CL
/
Pontificia Universidad Católica de Chile/CL
/
Universidad de Chile/CL
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