Patrón de metilación génico en el cáncer de mama / Promoter methylation profile in breast cancer
Rev. méd. Chile
;
132(9): 1069-1077, sept. 2004. tab
Article
in Spanish
| LILACS
| ID: lil-443219
ABSTRACT
BACKGROUND:
Genomic DNA methylation, mutations and allelic deletions explain the inactivation of genes involved in cell proliferation and cell cycle control mechanisms.AIM:
To analyze the methylation pattern of important genes related to different carcinogenic mechanisms in patients with breast cancer and the relationship with its biological behavior. MATERIAL ANDMETHODS:
Seventy fresh-frozen breast cancer samples were selected. The methylation specific PCR (MSP) test was used to analyze promoter methylation status for genes CDKN2A (p16), hMLH1, APC, CDH1 (Cadherin E) and FHIT.RESULTS:
We found methylation in at least one of the genes studied in 88% of cases and in 3 or more genes in 40.5% of cases. The frequencies of promoter hypermethylation of CDKN2A, hMLH1, APC, CDH1 and FHT were 41.4%, 11.4%, 52.9%, 70% and 42.9%, respectively. We found a relationship between CDKN2A methylatlon and better survival (p=0.002). CDH1 methylation and poor histological differentiation (p=0.007), hMLH1 methylation and non-Mapuche ethnicity (p=-0.03), APC methylation and larger tumor size (p<0.05), FHIT methylatton and lack of estrogen rectptor IHC expression (p<0.05).CONCLUSIONS:
The high frequency of promoter methylation in patients with breast cancer confirms its role in breast carcinogenesis. The finding of alterations in the methylation pattern of genes studied and its association with prognostic factors is a helpful tool in the search of new criteria for clinical and therapeutic decision making.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Breast Neoplasms
/
Genes, Tumor Suppressor
/
Promoter Regions, Genetic
/
Carcinoma, Ductal, Breast
/
DNA Methylation
Type of study:
Observational study
/
Prognostic study
/
Risk factors
Limits:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
Language:
Spanish
Journal:
Rev. méd. Chile
Journal subject:
Medicine
Year:
2004
Type:
Article
Affiliation country:
Chile
Institution/Affiliation country:
Universidad de La Frontera/CL
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