Cytoskeleton, endoplasmic reticulum and nucleus alterations in CHO-K1 cell line after Crotalus durissus terrificus (South American rattlesnake) venom treatment
J. venom. anim. toxins incl. trop. dis
;
13(1): 56-68, 2007. ilus
Article
in English
| LILACS
| ID: lil-444611
ABSTRACT
Snake venoms are toxic to a variety of cell types. However, the intracellular damages and the cell death fate induced by venom are unclear. In the present work, the action of the South American rattlesnake Crotalus durissus terrificus venom on CHO-K1 cell line was analyzed. The cells CHO-K1 were incubated with C. d. terrificus venom (10, 50 and 100g/ml) for 1 and 24 hours, and structural alterations of actin filaments, endoplasmic reticulum and nucleus were assessed using specific fluorescent probes and agarose gel electrophoresis for DNA fragmentation. Significant structural changes were observed in all analyzed structures. DNA fragmentation was detected suggesting that, at the concentrations used, the venom induced apoptosis.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
CHO Cells
/
Crotalid Venoms
/
Endoplasmic Reticulum
Limits:
Animals
Language:
English
Journal:
J. venom. anim. toxins incl. trop. dis
Journal subject:
Toxicology
Year:
2007
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
State University of Norte Fluminense/BR
/
Vale do Paraíba University/BR
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