Heterochromatin and chromosome evolution: a FISH probe of Cebus apella paraguayanus (Primate: Platyrrhini) developed by chromosome microdissection
Genet. mol. res. (Online)
;
4(4): 675-683, 2005. ilus
Article
in English
| LILACS
| ID: lil-444857
ABSTRACT
Neotropical Primate karyotypes are highly variable, particularly in the heterochromatic regions, not only regarding the amount of heterochromatin, but also the composition. G and C banding and FISH techniques provide useful information to characterize interspecific relationships. We used chromosome microdissection to develop a FISH probe of the chromosome 11 heterochromatic block (11qHe+) of Cebus apella paraguayanus (CAPp). Fragments of the 11qHe+ microdissected from fibroblast cell culture were collected in a PCR tube, amplified by degenerate oligonucleotide primer-PCR and subsequently labeled. The specificity of the FISH probe was confirmed in metaphases of some Ceboidea species. Signals were located in the He+ of chromosomes 4, 11, 12, 13, and 19 of CAPp and in the He+ of chromosomes 4, 12 and 13 of C. a. nigritus (CAPn); no signals were observed when other Ceboidea species were analyzed. We propose that the heterochromatin observed in CAPp and CAPn is specific for these species. We consider this C. apella heterochromatin identity as a possible key for the interpretation of chromosomal evolution in these Ceboidea.
Full text:
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Index:
LILACS (Americas)
Main subject:
Heterochromatin
/
Cebus
/
Chromosome Banding
/
In Situ Hybridization, Fluorescence
/
Evolution, Molecular
Type of study:
Diagnostic study
Limits:
Animals
Country/Region as subject:
South America
/
Paraguay
Language:
English
Journal:
Genet. mol. res. (Online)
Journal subject:
Molecular Biology
/
Genetics
Year:
2005
Type:
Article
Affiliation country:
Argentina
Institution/Affiliation country:
Comisión Nacional de Energía Atómica (CNEA)/AR
/
Universidad de Buenos Aires/AR
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