Detección y tipificación de virus papiloma humano en lesiones preneoplásicas del cuello uterino mediante PCR-RFLP / Detection and typification of human papilloma virus in pre cancerous cervical lesions
Rev. méd. Chile
;
135(2): 167-173, feb. 2007. ilus, tab
Article
in Spanish
| LILACS
| ID: lil-445055
ABSTRACT
Background:
The association of different genotypes of human papilloma virus (HPV) with cervical cancer is well known. However, there is little information about their association with pre-cancerous lesions.Aim:
To assess the frequency of different HPV genotypes in pre cancerous cervical lesions. Material andmethods:
A cervical sample was obtained by cytobrush in 15 women with low grade lesions and 40 women with high grade lesions, subjected to conization by loop electrical excision procedure (LEEP). Detection and typification of HPV was done by polymerase chain reaction and restriction fragment length polymorphism.Results:
All women were infected with HPV. Eighty five percent of samples were typified. A unique HPV subtype was found in 76 percent of women. Fourteen percent had an infection with multiple subtypes and in 10 percent, the viral genotype was not identified. The most common subtypes found were HPV 16, HPV 52 and HPV 53.Conclusions:
There is a high rate of infection with HPV with a high oncogenic risk among these women.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Papillomaviridae
/
Precancerous Conditions
/
Vaginal Smears
/
DNA, Viral
/
Uterine Cervical Dysplasia
/
Uterine Cervical Neoplasms
Type of study:
Diagnostic study
Limits:
Adolescent
/
Adult
/
Aged
/
Female
/
Humans
Language:
Spanish
Journal:
Rev. méd. Chile
Journal subject:
Medicine
Year:
2007
Type:
Article
/
Project document
Affiliation country:
Chile
Institution/Affiliation country:
Universidad de La Frontera/CL
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