Uses and limitations of two molecular cytogenetic techniques for the study of arrested embryos obtained through assisted reproduction technology
Genet. mol. res. (Online)
; Genet. mol. res. (Online);4(2): 143-151, 30 jun. 2005. tab, ilus
Article
in En
| LILACS
| ID: lil-445297
Responsible library:
BR1.1
ABSTRACT
We studied chromosomal abnormalities in arrested embryos produced by assisted reproductive technology with fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH) in order to determine the best technique for evaluating chromosomal aneusomies to be implemented in different situations. We examined individual blastomeres from arrested embryos by FISH and arrested whole embryos by CGH. All of the 10 FISH-analyzed embryos gave results, while only 7 of the 30 embryos analyzed by CGH were usable. Fifteen of the 17 embryos were chromosomally abnormal. CGH provided more accurate data for arrested embryos; however, FISH is the technique of choice for screening in preimplantation genetic diagnosis, because the results can be obtained within a day, while the embryos are still in culture.
Full text:
1
Index:
LILACS
Main subject:
In Situ Hybridization, Fluorescence
/
Preimplantation Diagnosis
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Chromosome Disorders
/
Genomics
/
Karyotyping
Type of study:
Diagnostic_studies
Limits:
Female
/
Humans
/
Pregnancy
Language:
En
Journal:
Genet. mol. res. (Online)
Journal subject:
BIOLOGIA MOLECULAR
/
GENETICA
Year:
2005
Type:
Article