Visualization of the ribosomal DNA (45S rDNA) of Indica rice with FISH on some phases of cell cycle and extended DNA fibers
Biocell
; Biocell;30(1): 27-32, abr. 2006. ilus
Article
in En
| LILACS
| ID: lil-448074
Responsible library:
AR40.1
ABSTRACT
The ribosomal DNA (45S rDNA) behaviors during the cell cycle were analyzed on interphase nuclei, prophases, metaphases, pachytene chromosomes and extended DNA fibers in rice (Oryza,sativa ssp.indica cv.Guangluai No.4) by using high-resolution fluorescent in situ hybridization (FISH). The results show that 45S rDNA is located at the ends of short arms of chromosomes 9 and 10. But the signals are much more intense on chromosome 9 than on chromosome 10 in metaphase. Pachytene chromosome has rDNA signal arrays on chromosome 9. Different phases are described and discussed. These results indicate that the activity of rDNA at individual loci may also vary through the cell cycle in rice. on extended DNA fibers, 45S rDNA signals appear as strings of numerous red spots, but some signals are missed in some regions, probably result from weak signals or intergenic spacers
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Index:
LILACS
Main subject:
Oryza
/
DNA, Ribosomal
/
Cell Cycle
/
In Situ Hybridization, Fluorescence
/
DNA, Plant
/
Chromosomes, Plant
Country/Region as subject:
Asia
Language:
En
Journal:
Biocell
Journal subject:
Clulas
Year:
2006
Type:
Article