Intramyocardial gene transfer of vascular endothelial growth factor 121 improves myocardial perfusion and function in the ischemic porcine heart
Electron. j. biotechnol
;
7(3): 08-09, Dec. 2004. ilus, graf, tab
Article
in English
| LILACS
| ID: lil-448765
ABSTRACT
Vascular endothelial growth factor (VEGF) is an endothelial cell-specific mitogen that is angiogenic in vitro and in vivo. Several studies report on gene transfer of VEGF121 to promote angiogenesis in the ischemic myocardium of animals and patients. We hypothesized that intramyocardial administration of naked plasmid DNA encoding VEGF121 could improve myocardial perfusion and function in a porcine model of myocardial ischemia. Yorkshire swine underwent thoracotomy and placement of an ameroid constrictor on the circumflex coronary artery. Four weeks later, pVEGF121 plasmid was administered into the ischemic myocardium. Four weeks after gene transfer, SPECT imaging demonstrated significant reduction in the ischemic area in pVEGF121-treated animals compared with controls. In the pVEGF121 group, most of the animals evolved from light ischemia to a normal perfusion. In contrast, control animals exhibited similar or impaired ischemic conditions. Our results indicate that intramyocardial gene transfer of VEGF121 as naked plasmid DNA results in significant improvement in myocardial perfusion and function.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Genetic Therapy
/
Myocardial Ischemia
/
Collateral Circulation
/
Vascular Endothelial Growth Factor A
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2004
Type:
Article
Affiliation country:
Cuba
Institution/Affiliation country:
Centro de Ingeniería Genética y Biotecnología/CU
/
Centro de Investigaciones Clínicas/CU
/
Hospital Hermanos Ameijeiras/CU
/
Hospital Salvador Allende/CU
/
Instituto de Investigaciones Porcinas/CU
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