Preliminary results of using ESR to examine biofilms
Electron. j. biotechnol
;
7(3): 11-12, Dec. 2004. ilus, tab, graf
Article
in English
| LILACS
| ID: lil-448768
ABSTRACT
This preliminary work shows ESR (Electron Spin Resonance) can be used to detect biofilms, particularly from Fe-metabolising bacteria. A film was detected by ESR as early as 1 day, hence possibly more sensitively than by fluorescent methods. Films can probably be detected as early as one hour. Spectra contain a very broad peak at g=2.13, probably due to ferrihydrite. Results of field experiments from streams and ponds in New Zealand and Japan, particularly the Minoh River, showed a general increase of ferrihydrite with time. Loss by exfoliation was later than 20 days. The rate of accumulation was faster in a nutrient-rich stagnant pond. Hematite (g=4.3) was often observed, magnetite (g=9) once, and usually small amounts of a common bacterial decay product. The latter was detected for at least 18 months film storage. ESR is a particularly good tool for observing the growth of oxic biofilms containing Fe-metabolising bacteria, and should be just as sensitive for observing Mn-metabolising bacteria in reducing conditions.
Full text:
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Index:
LILACS (Americas)
Main subject:
Bacteria
/
Ferric Compounds
/
Electron Spin Resonance Spectroscopy
/
Biofilms
/
Ferrosoferric Oxide
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2004
Type:
Article
Affiliation country:
Japan
Institution/Affiliation country:
Kanazawa University/JP
/
Osaka University/JP
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