Recombinant expression of marine shrimp lysozyme in Escherichia coli
Electron. j. biotechnol
;
7(3): 12-13, Dec. 2004. ilus, tab, graf
Article
in English
| LILACS
| ID: lil-448769
ABSTRACT
Shrimp Lysozyme (Lyz) is a key component of the antibacterial response as part of the innate defense in Crustacea; however, it has not been possible to purify this protein because of the very low amount present in the shrimp blood cells (hemocytes). In an effort to produce enough protein to study its function and biochemical properties we have overexpressed Lysozyme from marine shrimp (Penaeus vannamei) in E. coli. A bacterial protein expression system based on the T7 polymerase promoter was used. Although Lyz was produced as insoluble protein in inclusion bodies, its refolding led to an active protein with a yield of ~10 percent. Details of the protein recombinant expression techniques applied to this shrimp protein are presented.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Recombinant Proteins
/
Muramidase
/
Penaeidae
/
Escherichia coli
Limits:
Animals
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2004
Type:
Article
Affiliation country:
Mexico
Institution/Affiliation country:
Centro de Investigación en Alimentación y Desarrollo A.C/MX
/
Centro de Investigación en Alimentación y Desarrollo, A.C/MX
/
Universidad de Sonora/MX
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