Prevalencia de anticuerpos anti envoltura nuclear y sus isotipos en sueros positivos para anticuerpos antinucleares / Prevalence of antinuclear envelope antibodies and their isotypes in sera positive for antinuclear antibodies
Medicina (B.Aires)
;
66(4): 327-331, 2006. tab, ilus
Article
in Spanish
| LILACS
| ID: lil-449013
ABSTRACT
Antinuclear antibodies detected in HEp-2 cells by indirect immunofluorescence assay display a great variety of images, including the nuclear envelope pattern. This is quite a less frequent finding. Two thousand five hundred and ninety-four sera were processed, and 37.6% of ANA were detected. The prevalence of anti-nuclear envelope antibodies (ANEA) was of 1.2%, with a high association with autoimmune liver diseases (83%) and a low association with systemic lupus erythematosus. In 21 sera of patients with ANEA, no anti-DNAn antibodies were found; but 28.6% of anti-smooth muscle antibodies and 19% of anti-mitochondrial antibodies were detected. The triple rodent tissue section proved to be a less sensitive substrate than HEp-2 for the detection of ANEA. When using conjugates against different isotypes of antibodies for the detection of ANA, 90.5% of IgG, 66.6% of IgA and 9.5% of IgM. Two patients had ANEA-IgA at high titers (> or = 1160) without ANEA-IgG. In this work, the importance of performing complementary tests for the detection of anti-smooth muscle antibodies, anti-mitochondrial antibodies and anti-DNAn is highlighted in order to apply these tests as guidelines for the clinical diagnosis of patients with ANEA. Besides, this study expresses the need of using total anti-Ig antibodies as conjugate for IIF-HEp-2 instead of anti-lgG; until the role of IgA antibodies in these autoimmune diseases is clarified.
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Index:
LILACS (Americas)
Main subject:
Immunoglobulin Isotypes
/
Antibodies, Antinuclear
/
Nuclear Envelope
Type of study:
Practice guideline
/
Prevalence study
/
Prognostic study
/
Risk factors
/
Screening study
Limits:
Animals
/
Humans
Language:
Spanish
Journal:
Medicina (B.Aires)
Journal subject:
Medicine
Year:
2006
Type:
Article
Affiliation country:
Argentina
Institution/Affiliation country:
Universidad de Buenos Aires/AR
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