Analysis of slipped sequences in EST projects
Genet. mol. res. (Online)
;
5(1): 169-181, Mar. 31, 2006. ilus, graf, tab
Article
in English
| LILACS
| ID: lil-449135
ABSTRACT
Slippage is an important sequencing problem that can occur in EST projects. However, very few studies have addressed this. We propose three new methods to detect slippage artifacts arithmetic mean method, geometric mean method, and echo coverage method. Each method is simple and has two different strategies for processing sequences suffix and subsequence. Using the 291,689 EST sequences produced in the SUCEST project, we performed comparative tests between our proposed methods and the SUCEST method. The subsequence strategy is better than the suffix strategy, because it is not anchored at the end of the sequence, so it is more flexible to find slippage at the beginning of the EST. In a comparison with the SUCEST method, the advantage of our methods is that they do not discard the majority of the sequences marked as slippage, but instead only remove the slipped artifact from the sequence. Based on our tests the echo coverage method with subsequence strategy shows the best compromise between slippage detection and ease of calibration.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Genetic Techniques
/
Sequence Analysis, DNA
/
Expressed Sequence Tags
/
Saccharum
/
Models, Genetic
Limits:
Humans
Language:
English
Journal:
Genet. mol. res. (Online)
Journal subject:
Molecular Biology
/
Genetics
Year:
2006
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Scylla Bioinformática/BR
/
Unicamp/BR
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