A modified simple RFLP-PCR method for single nucleotide polymorphism (SNP) typing
Genet. mol. biol
;
29(3): 562-565, 2006. tab, ilus
Article
in English
| LILACS
| ID: lil-450299
ABSTRACT
We describe a modified single nucleotide polymorphism (SNP) typing method based on the restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR). This is a simple, economical method without the need for special equipment. For most SNP loci, a common restriction endonuclease (Hind III, EcoR I or BamH I) recognizing site (RER) can be introduced into one allelic form, but not the other by two rounds of mismatched PCR. The flanking regions can be changed by as many as five bases after PCR amplification with specially designed mismatching primers so the genotypes can be distinguished after digestion of the PCR products with corresponding endonucleases.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Polymorphism, Restriction Fragment Length
/
Polymorphism, Single Nucleotide
Language:
English
Journal:
Genet. mol. biol
Journal subject:
Genetics
Year:
2006
Type:
Article
/
Project document
Affiliation country:
China
Institution/Affiliation country:
East China University of Science and Technology/CN
/
Shanghai Bioengineering Research Center/CN
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