Your browser doesn't support javascript.
loading
Noninvasive genetic sampling of endangered muriqui (Primates, Atelidae): efficiency of fecal DNA extraction
Chaves, Paulo B; Paes, Marcela F; Mendes, Sérgio L; Strier, Karen B; Louro, Iúri D; Fagundes, Valéria.
  • Chaves, Paulo B; Universidade Federal do Espírito Santo. Departamento de Ciências Biológicas. Laboratório de Genética Animal. Vitória. BR
  • Paes, Marcela F; Universidade Federal do Espírito Santo. Departamento de Ciências Biológicas. Laboratório de Genética Animal. Vitória. BR
  • Mendes, Sérgio L; Universidade Federal do Espírito Santo. Departamento de Ciências Biológicas. Laboratório de Biologia da Conservação de Vertebrados. Vitória. BR
  • Strier, Karen B; University of Wisconsin-Madison. Department of Anthropology. Madison. US
  • Louro, Iúri D; Universidade Federal do Espírito Santo. Departamento de Ciências Biológicas. Núcleo de Genética Humana e Molecular. Vitória. BR
  • Fagundes, Valéria; Universidade Federal do Espírito Santo. Departamento de Ciências Biológicas. Laboratório de Genética Animal. Vitória. BR
Genet. mol. biol ; 29(4): 750-754, 2006. ilus
Article in English | LILACS | ID: lil-450501
ABSTRACT
The muriqui (Brachyteles) is one of the most endangered primates in the world, however little is known about the viability of the remaining populations. We evaluated the technique of extracting DNA from wild muriqui feces for PCR applications. In order to determine the effect of the DNA in subsequent amplifications, we analyzed five different extracts. The importance of the recommended BSA and the HotStarTaq DNA polymerase was tested. The minimal conditions to successfully amplify highly degraded fecal DNA were determined, showing that the recommended reagents are not required. We envision that this method may be useful in further conservation management studies.
Subject(s)
Fulltext
Print
XML
Search on Google
Full text: Available Index: LILACS (Americas) Main subject: Primates / DNA / Feces Limits: Animals / Humans Language: English Journal: Genet. mol. biol Journal subject: Genetics Year: 2006 Type: Article Affiliation country: Brazil / United States Institution/Affiliation country: Universidade Federal do Espírito Santo/BR / University of Wisconsin-Madison/US

Similar

MEDLINE
LILACS
LIS
Fulltext
Print
XML
Search on Google
Full text: Available Index: LILACS (Americas) Main subject: Primates / DNA / Feces Limits: Animals / Humans Language: English Journal: Genet. mol. biol Journal subject: Genetics Year: 2006 Type: Article Affiliation country: Brazil / United States Institution/Affiliation country: Universidade Federal do Espírito Santo/BR / University of Wisconsin-Madison/US