Avaliação eletrofisiológica in vitro de drogas antiepilépticas em fatias hipocampais humanas provenientes de pacientes portadores de epilepsia do lobo temporal refratária ao tratamento medicamentoso / In vitro electrophysiological assessment of antiepileptic drugs in human hippocampal slices originating from patients with refractory temporal lobe epilepsy
J. epilepsy clin. neurophysiol
; 12(2): 73-74, June 2006.
Article
in Pt
| LILACS
| ID: lil-451667
Responsible library:
BR1.1
RESUMO
The absence of a satisfactory response to antiepileptic drug (AED) therapy, is an unresolved problem in a significant number of epileptic patients. Mechanisms of intractability are not well understood but may include a combination of poor penetration of AED across a functionally altered blood-brain barrier owing to increased expression of multiple drug resistance transporters. Therefore, the aim of this work was to assess the in vitro efficacy of antiepileptic drugs through human hippocampal slices originating from patients with refractory temporal lobe epilepsy submitted to corticoamygdalohippocampectomy. Slices was prepared from a 1 cm3 block of the hippocampus body 30 min after resection. Briefly, hippocampal slices of 400 µM thickness was cut coronally. Extracellular field potentials was recorded from the st. Granulosum of the dentate gyrus. The antiepileptic drugs added in the bath were Carbamazepine, Topiramate and Phenytoin. The phenytoin was effective reducing the hyperexcitability (polispikes) in 60% of the experiments (n = 5). On the other hand, the carbamazepine promoted a decrease in evoked epileptiform activity in 37,5% of the cases (n = 8). The application of topiramate in the bath reduced in 30% the number of polispikes (n = 10). Our results showed that the phenytoin application resulted in a significant reduction in neuronal excitability, however, the carbamazepine and topiramate were not able to control of the hiperexcitability, suggesting that local neuronal alterations, as well changes in blood brain barrier, could be responsible for such behaviors.
Key words
Full text:
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Index:
LILACS
Main subject:
Sclerosis
/
Electrophysiology
/
Epilepsy, Temporal Lobe
/
Anticonvulsants
Limits:
Humans
Language:
Pt
Journal:
J. epilepsy clin. neurophysiol
Journal subject:
NEUROCIENCIAS
Year:
2006
Type:
Article