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Quantitative real-time PCR for the clinical detection of Helicobacter pylori
Ribeiro, Marcelo Lima; Ecclissato, Christina Cunha; Mattos, Ricardo Gabriel; Mendonca, Sergio; Pedrazzoli Júnior, José.
  • Ribeiro, Marcelo Lima; Universidade São Francisco. Unidade Integrada Farmacologia e Gastroenterologia. Bragança Paulista. BR
  • Ecclissato, Christina Cunha; Universidade São Francisco. Unidade Integrada Farmacologia e Gastroenterologia. Bragança Paulista. BR
  • Mattos, Ricardo Gabriel; Universidade São Francisco. Unidade Integrada Farmacologia e Gastroenterologia. Bragança Paulista. BR
  • Mendonca, Sergio; Universidade São Francisco. Unidade Integrada Farmacologia e Gastroenterologia. Bragança Paulista. BR
  • Pedrazzoli Júnior, José; Universidade São Francisco. Unidade Integrada Farmacologia e Gastroenterologia. Bragança Paulista. BR
Genet. mol. biol ; 30(2): 431-434, Mar. 2007. graf
Article in English | LILACS | ID: lil-452823
ABSTRACT
Accurate diagnosis of Helicobacter pylori infection is very important in both clinical practice and research. We evaluated the sensitivity of real-time PCR (RT-PCR) for the detection and quantification of Helicobacter pylori using DNA from 91 human gastric biopsy samples divided into three groups 46 biopsies from untreated patients who according to the references methods were considered H. pylori-negative (group A); 35 biopsies from patients previously treated against H. pylori and considered to be cured by "gold standard" tests (group B); and 10 biopsies from patients H. pylori-positive by all available methods (group C). The sensitivity of the RT-PCR assay was higher than that of standard methods. Of the 81 patients considered to be uninfected according to the references methods, 16 were H. pylori-positive by PCR, 10 of which were patients who had received H. pylori eradication therapy and 6 were untreated patients. Based on these findings we recommend that RT-PCR should be use in addition to standard methods in clinical studies to monitor the results of H. pylori eradication therapy.
Full text: Available Index: LILACS (Americas) Type of study: Diagnostic study Language: English Journal: Genet. mol. biol Journal subject: Genetics Year: 2007 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade São Francisco/BR

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Full text: Available Index: LILACS (Americas) Type of study: Diagnostic study Language: English Journal: Genet. mol. biol Journal subject: Genetics Year: 2007 Type: Article Affiliation country: Brazil Institution/Affiliation country: Universidade São Francisco/BR