Analysis of the association between Epstein-Barr virus and classic Hodgkins lymphoma in adult patients from Ceará (Brazil) by immunohistochemistry and in situ hybridization
J. bras. patol. med. lab
;
42(3): 201-205, jun. 2006. ilus, tab
Article
in English
| LILACS
| ID: lil-453002
ABSTRACT
The prevalence of Epstein-Barr virus (EBV) in patients with classical Hodgkins lymphoma (CHL) is geographically variable. In the present study the prevalence of EBV in CHL was assessed in adult patients from Ceará, Brazil. Thirty-seven cases were immunohistochemically evaluated for EBV using latent membrane protein (LMP1) antibody and for EBV latency-associated RNA (EBER1) using in situ hybridization (ISH). Sex and age did not differ among patients as to the frequency of CHL. Nodular sclerosis was the predominant histological subtype. LMP1 was found in Reed-Sternberg cells in 67.5 percent of the cases whereas ISH detected EBER1 in 75.6 percent. Regarding histological subtypes EBV infection rates were not found statistically different in nodular sclerosis (NS) and mixed cellularity (MC) subtypes (p = 0.66).
RESUMO
A freqüência do vírus Epstein-Barr (EBV) em pacientes com linfoma de Hodgkin Clássico (LHC) sofre variabilidade geográfica. No presente estudo investigamos a freqüência do EBV em pacientes com LHC no estado do Ceará. Trinta e sete casos de linfoma de Hodgkin clássico foram avaliados por imuno-histoquímica para EBV usando o anticorpo monoclonal contra a proteína latente da membrana (LMP1) e pelo método de hibridização in situ para RNA associado ao EBV (EBER1). Não há diferença por sexo e idade dos pacientes no que concerne à freqüência de LHC. O subtipo histológico esclerose nodular foi predominante. LMP1 esteve presente em células Reed-Sternberg em 67,5 por cento e pela hibridização in situ, através da sonda EBER, foi evidente em 75,6 por cento dos casos. Não observamos predominância significativa da associação de EBV com os subtipos histológicos esclerose nodular (EN) e celularidade mista (CM) (p = 0,66).
Full text:
Available
Index:
LILACS (Americas)
Main subject:
DNA, Viral
/
Hodgkin Disease
/
Immunohistochemistry
/
In Situ Hybridization
/
Epstein-Barr Virus Infections
Type of study:
Risk factors
Limits:
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
J. bras. patol. med. lab
Journal subject:
Pathology
Year:
2006
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
UFC/BR
/
Universidade Estadual do Ceará/BR
/
Universidade Federal do Ceará/BR
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