Identification and characterization of previously described epitopes in HIV-1 subtypes B, C, F and BF in Brazil
Braz. j. infect. dis
;
11(1): 27-30, Feb. 2007. tab
Article
in English
| LILACS
| ID: lil-454679
ABSTRACT
Genetic analysis of HIV-1 is essential to improve treatment strategies and select epitopes for vaccine programs. The objective of this study was to determine whether known CD4+ and CD8+ epitopes were present in Brazilian HIV-1 strains. We used previously described CD8+ and CD4+ epitopes from the Los Alamos laboratory to search for these epitopes in the Brazilian sequences using the HIVbase program and we compared the frequency results with the analyses using physical-chemical profile tools from Network Protein Sequence Analysis (NPSA), and the SYFPEITHI program. Furthermore, this analysis was carried out with the Prosite tool using the GeneDoc program and ds/dn analyses using the Synonymous Nonsynonymous Analysis Program (SNAP). The HIVbase epitope mapping demonstrated that 30 CD8+ and 6 CD4+ epitopes were present in the Brazilian sequences at a high frequency. Only two of these epitopes were heavily glycosylated. Interestingly, ds/dn analyses showed evidence of purifying selective pressure. These types of analyses could be useful for the assessment of possible vaccine efficiency in populations.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Gene Products, env
/
HIV Infections
/
HIV-1
/
Epitopes
Type of study:
Diagnostic study
Limits:
Humans
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Braz. j. infect. dis
Journal subject:
Communicable Diseases
Year:
2007
Type:
Article
/
Project document
Affiliation country:
Brazil
/
United States
/
United kingdom
Institution/Affiliation country:
Oswaldo Cruz Foundation/BR
/
University of Oxford/GB
/
University of Wisconsin Medical School/US
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