Trypanosoma cruzi ribosomal protein S4: characterization of its coding locus, analysis of transcripts, and antigenicity of the protein
Mem. Inst. Oswaldo Cruz
;
102(4): 473-479, June 2007. ilus
Article
in English
| LILACS
| ID: lil-454799
ABSTRACT
Two allelic genomic fragments containing ribosomal protein S4 encoding genes (rpS4) from Trypanosoma cruzi (CL-Brener strain) were isolated and characterized. One allele comprises two complete tandem repeats of a sequence encoding an rpS4 gene. In the other, only one rpS4 gene is found. Sequence comparison to the accessed data in the genome project database reveals that our two-copy allele corresponds to a variant haplotype. However, the deduced aminoacid sequence of all the gene copies is identical. The rpS4 transcripts processing sites were determined by comparison of genomic sequences with published cDNA data. The obtained sequence data demonstrates that rpS4 genes are expressed in epimastigotes, amastigotes, and trypomastigotes. A recombinant version of rpS4 was found to be an antigenic it was recognized by 62.5 percent of the individuals with positive serology for T. cruzi and by 93.3 percent of patients with proven chronic chagasic disease.
Full text:
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Index:
LILACS (Americas)
Main subject:
Ribosomal Proteins
/
Trypanosoma cruzi
/
Chagas Disease
Type of study:
Observational study
Limits:
Animals
/
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2007
Type:
Article
Affiliation country:
Mexico
Institution/Affiliation country:
Universidad Nacional Autónoma de México/MX
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