Asexual Recombination in a uvsH Mutant of Aspergillus nidulans
Biol. Res
;
40(1): 65-71, 2007. ilus, tab, graf
Article
in English
| LILACS
| ID: lil-456609
ABSTRACT
Mutations in the gene uvsH of Aspergillus nidulans result in increased spontaneous chromosome instability and increased intragenic and intergenic mitotic recombination in homozygous diploids. The aim of the present work was to obtain a uvs mutant of A. nidulans and to use it for the isolation of asexual recombinants (parameiotic segregants). The mutant uvsH, named B511, showed normal frequency of meiotic recombination in sexual crosses and high frequency of parameiotic segregants in the parasexual crossings with master strains (B511//A757 and B511//A288). Asexual haploid recombinants (parameiotic segregants), diploid and aneuploid segregants were recovered directly from the uvs//uvs+ heterokaryons (B511//A757 and B511// A288). Parameiotic segregants originated through mitotic crossing-over and independent assortment of chromosomes.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Aspergillus nidulans
/
Reproduction, Asexual
/
Crossing Over, Genetic
/
Genes, Fungal
/
Mutation
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2007
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
State University of Maringá/BR
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