Aumento del daño en el ADN y estrés oxidativo en espermatozoides de pacientes con oligozoospermia idiopática y antecedentes de criptorquidismo / Extent of sperm DNA damage in spermatozoa from men examined for infertility. Relationship with oxidative stress
Rev. méd. Chile
;
135(3): 279-286, mar. 2007. graf, tab
Article
in Spanish
| LILACS
| ID: lil-456612
ABSTRACT
Background:
Cryptorchidism and oligozoospermia are clinical conditions closely associated with impaired fertility. Oxidative stress and related sperm DNA damage have been identified as significant causes of male infertility.Aim:
To determine the extent of sperm nuclear DNA damage in patients affected with idiopathic oligozoospermia or undescended testes and to examine its relationship with oxidative stress. Patients andmethods:
We studied 20 patients with idiopathic oligozoospermia and 18 with undescended testes (who previously underwent orchiopexy) and 25 normozoospermic healthy controls. All subjects underwent semen analysis. Sperm DNA damage was evaluated by the sperm chromatin structure assay/flow cytometry (SCSA-FCM) and by the dUTP-biotin nick end labeling (TUNEL) assay. Levels of reactive oxygen species (ROS) and total antioxidant capacity (TAC) were assessed by a chemiluminescence assay.Results:
DFI (percentage of sperm with denatured DNA) values and percentage of TUNEL positive cells were significantly greater in patients with oligozoospermia (DFI 28.8±5.6; TUNEL+ 26.9±3.0) or cryptorchidism (DFI 26.4±10.1; TUNEL+ 29.1±3.9), compared with controls (DFI 7.1±0.9; TUNEL+ 14.2±1.2). Similarly, both groups of patients had significantly higher (p <0.01) levels of ROS. TAC levels did not differ between control and patient groups, suggesting that the DNA damage occurs before spermiation.Conclusions:
Sperm DNA damage is significantly increased in men with idiopathic oligozoospermia and in cryptorchid subjects. The finding of increased ROS levels may indicate that seminal oxidative stress may be involved in the pathogenesis of sperm DNA damage in these patients.
Full text:
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Index:
LILACS (Americas)
Main subject:
Spermatozoa
/
DNA Damage
/
Chromatin
/
Oxidative Stress
/
Infertility, Male
Type of study:
Observational study
/
Prognostic study
/
Risk factors
Limits:
Adult
/
Humans
/
Male
Language:
Spanish
Journal:
Rev. méd. Chile
Journal subject:
Medicine
Year:
2007
Type:
Article
/
Project document
Affiliation country:
Chile
Institution/Affiliation country:
Hospital Parroquial de San Bernardo/CL
/
Universidad Diego Portales/CL
/
Universidad de Chile/CL
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