Estudo da camada elástica das arteríolas pulmonares em portadores de granulomatose de Wegener / Study of the elastic fibers of the pulmonary arteriolas of patients with Wegener granulomatosis

RESUMO

A Granulomatose de Wegener é uma síndrome clínico-patológica caracterizada por vasculite granulomatosa necrozante que acomete o trato respiratório superior, pulmões e rins. A vasculite granulomatosa afeta os vasos pulmonares com destruição das camadas elásticas das artérias.. O objetivo do presente estudo foi quantificar o sistema elástico da parede das artérias pulmonares de pacientes portadores de Granulomatose de Wegener comparado com o de pacientes normais. Foram estudadas 36 artérias de pequeno calibre ( diâmetro < 62 micra) e 36 artérias de médio calibre ( diâmetro entre 62 e 213 micra) obtidas de tecido pulmonar de 6 pacientes portadores de Granulomatose de Wegener comparadas com 36 artérias de pequeno e 36 artérias de médio calibre obtidas de 6 pacientes normais...

ABSTRACT

Wegener Granulomatosis is a clinico-pathologic syndrome characterized by necrotizing granulomatous vasculitis that affects the upper respiratory tract, the lungs and the kidneys. The granulomatous vasculitis affects the pulmonary vessels with destruction of the elstic system fibers of the vessels walls that can be visualized with the Verhoff staining technique . The objective of the present study was to quantify the area of the elastic system fibers of the walls of the pulmonary arteries of patients with the diagnosis of Wegener Granulomatosis compared to the area of the elastic system fibers of normal patients. We studied 36 small size pulmonary arteries ( diameter < 62 µ) and 36 medium size pulmonary arteries ( diameter between 62 and 213µ) taken from lung tissue of 6 patients with the diagnosis of Wegener Granulomatosis compared to 36 small size and 36 medium size pulmonary arteries taken from lung tissue of normal patients. The elastic system fibers of the pulmonary arteries walls was atained by resorcine-fucsine technique e the área of the elastic system was quantified through a special softer for área calculation of the Image analyser FOX pro of the Faculty of Medicine of the University of São Paulo. The medium area of the elastic system fibers of the small size pulmonary arteries of Wegener Granulomatosis was 73,6 ± 20,9 µ² ( 29,76 to 109,05 µ²) compared to 680,8 ± 256,1 µ² ( 263,34 to 1380,75 µ²) of the normal patients ( p< 0,0001- ANOVA -2 factors)...