Polymerase chain reaction with two molecular targets in mucosal leishmaniasis' diagnosis: a validation study
Mem. Inst. Oswaldo Cruz
;
102(5): 549-554, Aug. 2007. tab, ilus
Article
in English
| LILACS
| ID: lil-458620
ABSTRACT
We validated the polymerase chain reaction (PCR) with a composite reference standard in 61 patients clinically suspected of having mucosal leishmaniasis, 36 of which were cases and 25 were non-cases according to this reference standard. Patient classification and test application were carried out independently by two blind observers. One pair of primers was used to amplify a fragment of 120 bp in the conserved region of kDNA and another pair was used to amplify the internal transcript spacers (ITS) rDNA. PCR showed 68.6 percent (95 percent CI 59.2-72.6) sensitivity and 92 percent (95 percent CI 78.9-97.7) specificity; positive likelihood ratio 8.6 (95 percent CI 2.8-31.3) and negative likelihood ratio 0.3 (95 percent CI 0.3-0.5), when kDNA molecular target was amplified. The test performed better on sensitivity using this target compared to the ITS rDNA molecular target which showed 40 percent (95 percent CI 31.5-42.3) sensitivity and 96 percent (95 percent CI 84.1-99.3) specificity; positive likelihood ratio 10 (95 percent CI 2.0-58.8) and negative likelihood ratio 0.6 (95 percent CI 0.6-0.8). The inter-observer agreement was excellent for both tests. Based upon results obtained and due to low performance of conventional methods for diagnosing mucosal leishmaniasis, we consider PCR with kDNA as molecular target is a useful diagnostic test and the ITS rDNA molecular target is useful when the aim is to identify species.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Leishmania braziliensis
/
Leishmaniasis, Mucocutaneous
/
Polymerase Chain Reaction
Type of study:
Diagnostic study
/
Observational study
/
Prognostic study
/
Risk factors
Limits:
Animals
/
Female
/
Humans
/
Male
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2007
Type:
Article
Affiliation country:
Colombia
Institution/Affiliation country:
Centro Dermatológico Federico Lleras Acosta/CO
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