Isolation and identification of bovine tuberculosis in a Brazilian herd (São Paulo)
Mem. Inst. Oswaldo Cruz
;
102(5): 639-642, Aug. 2007. tab
Article
in English
| LILACS
| ID: lil-458627
ABSTRACT
Mycobacterium was verified in animals from a Brazilian dairy herd, a total of 42 samples from 30 cows were submitted to culture and the isolated strains were analyzed by two polymerase chain reaction (PCR), the first specific for species belonging to the Mycobacterium complex (MTBC) and the other for differentiating M. tuberculosis from M. bovis. Twenty seven samples (64.3 percent) from 18 animals (60 percent) were positive for mycobacteria by culture, including samples from 15 retrofaryngeal lymphnodes (55.5 percent), 9 prescapular lymphnodes (33.3 percent), 2 lungs (7.4 percent), and 1 liver (3.7 percent). All isolated colonies were confirmed by PCR to contain MTBC organisms, and were identified as M. bovis by the same methodology.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Tuberculosis, Bovine
/
Mycobacterium bovis
/
Mycobacterium tuberculosis
Type of study:
Diagnostic study
Limits:
Animals
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2007
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Coordenadoria de Assistência Técnica Integral/BR
/
Instituto Biológico/BR
/
Universidade de São Paulo/BR
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