Isolation and molecular identification of Leishmania ( Viannia ) peruviana from naturally infected Lutzomyia peruensis (Diptera: Psychodidae) in the Peruvian Andes
Mem. Inst. Oswaldo Cruz
;
102(5): 655-658, Aug. 2007. ilus
Article
in English
| LILACS
| ID: lil-458635
ABSTRACT
Leishmania (Viannia) peruviana was isolated from 1/75 Lutzomyia peruensis captured during May 2006 in an endemic cutaneous leishmaniasis region of the Peruvian Andes (Chaute, Huarochiri, Lima, Peru). Sand fly gut with promastigotes was inoculated into a hamster and the remaining body was fixed in ethanol. L. (Viannia) sp. was determined by polymerase chain reaction (PCR), and Leishmania species through molecular genotyping by PCR-restriction fragment length polymorphism analyses targeting the genes cpb and hsp70, resulting L. (V.) peruviana. The infected sand fly appeared 15 days after the rains finished, time expected and useful real time data for interventions when transmission is occurring.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Psychodidae
/
Leishmania braziliensis
/
DNA, Protozoan
Type of study:
Diagnostic study
Limits:
Animals
Country/Region as subject:
South America
/
Peru
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2007
Type:
Article
Affiliation country:
Peru
Institution/Affiliation country:
Universidad Peruana Cayetano Heredia/PE
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