Polymorphism of exon 2-3 of bovine major histocompatibility complex class I BoLa-A gene
Genet. mol. biol
;
30(3): 560-566, 2007. ilus, tab
Article
in English
| LILACS
| ID: lil-460070
ABSTRACT
The exon 2-3 region of bovine major histocompatibility complex (MHC) class I BoLa-A gene was investigated for polymorphisms in three breeds of cattle originated in the Indian subcontinent namely Sahiwal, Tharparkar, Hariana, as well as crossbred (Bos taurus x Bos indicus) cattle and Jersey, the exotic breed (Bos taurus). The PCR amplified fragment of 714 bp showed distinct DdeI-, TaqI- and HinfI- RFLP patterns, thus confirming a higher degree of polymorphism in this region. To our knowledge this is the first report of HinfI restriction patterns for BoLa-A exon 2-3. The sequencing results revealed a number of nucleotide substitutions in this region, which resulted in amino acid changes. The present investigation confirmed that MHC class I BoLa-A exon 2-3 is highly polymorphic in cattle.
Full text:
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Index:
LILACS (Americas)
Language:
English
Journal:
Genet. mol. biol
Journal subject:
Genetics
Year:
2007
Type:
Article
Affiliation country:
India
Institution/Affiliation country:
Indian Veterinary Research Institute/IN
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