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Modo de herencia de la resistencia a temefos (abate) en Aedes aegypti (Diptera: Culicidae) de Cuba / Mode of inheritance of temephos (Abate) resistance in Aedes aegypti (Diptera: Culicidae) from Cuba
Rodríguez, María Magdalena; Bisset, Juan A; Pérez, Omayda; Ramos, Francisco; Risco, Grisel E.
  • Rodríguez, María Magdalena; Instituto de Medicina Tropical Pedro Kourí. CU
  • Bisset, Juan A; Instituto de Medicina Tropical Pedro Kourí. CU
  • Pérez, Omayda; Instituto de Medicina Tropical Pedro Kourí. CU
  • Ramos, Francisco; Instituto de Medicina Tropical Pedro Kourí. CU
  • Risco, Grisel E; Instituto de Medicina Tropical Pedro Kourí. CU
Rev. cuba. med. trop ; 58(2): 142-147, mayo-ago. 2006. ilus, graf
Article in Spanish | LILACS | ID: lil-460743
RESUMEN
Se realizó el estudio sobre el modo de herencia de la resistencia al insecticida organofosforado temefos, a partir de una cepa de Aedes aegypti de referencia (SAN-F6), resistente, con un valor de factor de resistencia de 200x, comparado con la cepa de Aedes aegypti susceptible a insecticidas (ROCKEFELLER). Se llevaron a cabo cruces genéticos entre la cepa resistente y la susceptible a temefos, obteniéndose una F1 (cruce). Las hembras de esta F1 del cruzamiento se cruzaron con machos de la cepa ROCKEFELLER (retrocruce) y como resultado se obtuvo que la resistencia a temefos se heredó de forma semidominante, mediada por un solo factor. La actividad de Est-A4, observada en la electroforesis en gel de poliacrilamida y medida mediante ensayos bioquímicos, fue mayor en la cepa resistente a temefos (SAN-F6), menor en la cepa susceptible (ROCKEFELLER) e intermedia en el cruce de estas 2 cepas. Un menor efecto del parental resistente fue observado en el retrocruce tanto en la mortalidad con temefos, como en la actividad de la Est. A4. Estos resultados sugieren que la actividad de esterasas pudiera heredarse también, al igual que la resistencia a temefos, como un carácter semidominante
ABSTRACT
A study on the mode of inheritance of temephos resistance was conducted using a temephos resistant Aedes aegypti reference strain (SAN-F6) with a value of resistance factor of 200x, compared with the insecticides susceptible Aedes aegypti strain (ROCKEFELLER). Genetic crossings were performed between temephos resistant and susceptible strains. An F1 crossing was attained. The females of this F1 crossing were crossed with males from the ROCKEFELLER strain (retrocrossing), and it was found that the temephos resistance was inherited in a semidominant way and as a monofactorial trait. The activity of Est-A4 observed in the polyacrylamide gel electrophoresis and measured by biochemical assays was higher in the strain resistant to temephos (SAN-F6), lower in the susceptible strain (ROCKEFELLER), and intermediate in the crossing of these two strains. A lower effect of the resistant parental strain was observed in the retrocrossing, both in the mortality with temephos and in the activity of Est. A4. These results suggest that the esterase activity may also be inherited, as well as the resistance to temephos, as a semidominant character.
Subject(s)
Full text: Available Index: LILACS (Americas) Main subject: Temefos / Aedes Country/Region as subject: Cuba Language: Spanish Journal: Rev. cuba. med. trop Journal subject: Tropical Medicine Year: 2006 Type: Article Affiliation country: Cuba Institution/Affiliation country: Instituto de Medicina Tropical Pedro Kourí/CU

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Full text: Available Index: LILACS (Americas) Main subject: Temefos / Aedes Country/Region as subject: Cuba Language: Spanish Journal: Rev. cuba. med. trop Journal subject: Tropical Medicine Year: 2006 Type: Article Affiliation country: Cuba Institution/Affiliation country: Instituto de Medicina Tropical Pedro Kourí/CU