Ochratoxin A in raisins samples marketed in São Paulo, Brazil
Rev. Inst. Adolfo Lutz
;
65(3): 171-175, set.-dez. 2006. tab, graf
Article
in English
| LILACS, SES-SP, SESSP-CTDPROD, SES-SP, SESSP-IALPROD, SES-SP
| ID: lil-461272
ABSTRACT
The present study aimed to evaluate a methodology for analyzing ochratoxin A (OTA) in raisins samples marketed in São Paulo, Brazil. OTA was extracted with methanol water (8020, v/v) and diluted with phosphate buffer solution, and purified through an immunoaffinity column. OTA was separated and quantified using HPLC with fluorescence detection. The established detection and quantification limits were 0.24 and 0.80 ng/g, respectively. The recoveries values were 81.6, 80.4 and 81.9%, and the relative standard deviations (RSD) were 6.0, 4.3, and 6.1 % at 2.0, 5.0 and 10.0 ng/g levels, respectively. Of twenty black raisin samples analyzed 10 (50 %) contained OTA at levels ranging from 1.3 to 39.1 ng/g. All of twenty-two white raisin samples showed no contamination with OTA.
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Index:
LILACS (Americas)
Main subject:
Brazil
/
Vitis
/
Ochratoxins
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Rev. Inst. Adolfo Lutz
Year:
2006
Type:
Article
Institution/Affiliation country:
Instituto Adolfo Lutz/BR
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