Evaluation of oxacillin and cefoxitin disks for detection of resistance in coagulase negative staphylococci
Mem. Inst. Oswaldo Cruz
;
102(6): 719-723, Sept. 2007. ilus, tab
Article
in English
| LILACS
| ID: lil-463478
ABSTRACT
Coagulase-negative Staphylococcus spp. was considered nonpathogenic until the emergence of multiresistance and the demonstration of their participation as infectious agents. In Brazil, oxacillin resistance may be present in over 80 percent of isolates, and the Clinical and Laboratory Standards Institute standardized a disk-diffusion method to predict this resistance in Staphylococcus. The aim of this study was to evaluate the variability among commercial disks of oxacillin (1 mug) and cefoxitin (30 mug) widely used in clinical laboratories of microbiology, compared with mecA gene and minimum inhibitory concentration (MIC) of oxacillin. The use of oxacillin and cefoxitin disks simultaneously allowed the detection of important differences, particularly, in less frequent species such as S. cohnii, S. haemolyticus, S. saprophyticus, and S. sciuri. Disks of cefoxitin of the brand 2 displayed good correlation with the mecA gene (98.7 percent) and oxacillin MIC (97.8 percent), while major discrepancies were observed using disks of brand 1. One of the critical points in the diffusion disk test is the quality of the disks the use of better quality disks associated with molecular methods lead to better results to define the best antibiotic therapy.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Oxacillin
/
Staphylococcus
/
Cefoxitin
/
Methicillin Resistance
/
Anti-Bacterial Agents
Type of study:
Diagnostic study
/
Evaluation studies
/
Practice guideline
/
Prognostic study
Limits:
Humans
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2007
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Fundação Faculdade Federal de Ciências Médicas de Porto Alegre/BR
/
Universidade Federal do Rio Grande do Sul/BR
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