Effects of Trypanosoma brucei tryptophanyl-tRNA synthetases silencing by RNA interference
Mem. Inst. Oswaldo Cruz
;
102(6): 757-762, Sept. 2007. ilus, graf, tab
Article
in English
| LILACS
| ID: lil-463485
ABSTRACT
The kinetoplast genetic code deviates from the universal code in that 90 percent of mitochondrial tryptophans are specified by UGA instead of UGG codons. A single nucleus-encoded tRNA Trp(CCA) is used by both nuclear and mitochondria genes, since all kinetoplast tRNAs are imported into the mitochondria from the cytoplasm. To allow decoding of the mitochondrial UGA codons as tryptophan, the tRNA Trp(CCA) anticodon is changed to UCA by an editing event. Two tryptophanyl tRNA synthetases (TrpRSs) have been identified in Trypanosoma brucei TbTrpRS1 and TbTrpRS2 which localize to the cytoplasm and mitochondria respectively. We used inducible RNA interference (RNAi) to assess the role of TbTrpRSs. Our data validates previous observations of TrpRS as potential drug design targets and investigates the RNAi effect on the mitochondria of the parasite.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Trypanosoma brucei brucei
/
Tryptophan-tRNA Ligase
/
RNA, Transfer
/
RNA, Protozoan
/
RNA Interference
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Mem. Inst. Oswaldo Cruz
Journal subject:
Tropical Medicine
/
Parasitology
Year:
2007
Type:
Article
/
Project document
Affiliation country:
Brazil
/
United States
Institution/Affiliation country:
Ohio State University RNA Group/US
/
Universidade de São Paulo/BR
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