Determination of optimum wavelength and derivative order in spectrophotometry for quantitation of hydroquinone in creams
RBCF, Rev. bras. ciênc. farm. (Impr.)
;
43(3): 397-404, jul.-set. 2007. graf, tab
Article
in English
| LILACS
| ID: lil-468146
ABSTRACT
UV derivative spectrophotometry was used for quantitative determination of hydroquinone in creams. The aim of this work was to investigate optimum wavelength and order of derivative, and to validate the proposed spectrophotometric method. The results of standard curves were calculated and statistically analyzed through the least squares method in the interval from 10.0 to 26.0 µg/mL, in the first, second, third and fourth order derivatives. The quantitative determination was carried out by using the zero-crossing (Z-C) and zero-peak (Z-P) methods. The proposed method is simple, of low cost and provides reliable results in order to be used in quality control of creams containing hydroquinone as active substance.
RESUMO
A espectrofotometria derivada no UV foi usada para a determinação quantitativa de hidroquinona em cremes. O objetivo desta pesquisa foi investigar o melhor comprimento de onda e a ordem da derivada, bem como validar o método proposto. Os resultados das curvas analíticas foram analisados estatisticamente pelo método dos mínimos quadrados no intervalo de 10,0 a 26,0 µg/mL, na primeira, segunda, terceira e quarta ordens da derivada. As determinações quantitativas foram realizadas utilizando os métodos "zero-crossing (Z-C)" e zero-pico (Z-P). O método proposto é simples, de baixo custo e fornece resultados confiáveis podendo ser usado no controle de qualidade de cremes contendo hidroquinona como substância ativa.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Hydroquinones
Language:
English
Journal:
RBCF, Rev. bras. ciênc. farm. (Impr.)
Journal subject:
Biochemistry
/
Pharmacy
/
Pharmacology
Year:
2007
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
University of São Paulo/BR
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