The chromatin structure of the lysozyme GAS41 origin of DNA replication changes during the cell cycle
Biol. Res
;
40(2): 185-192, 2007. ilus
Article
in English
| LILACS
| ID: lil-468189
ABSTRACT
We used a rapid and simple protocol using lysolecithin for mapping HS sites in vivo. The protocol is based on partial digestion with DNase I of exponentially growing cells following permeabilization by short treatment with lysolecithin. Using this protocol, we analyzed the chromatin structure of the region surrounding two overlapping elements, an origin of bidirectional DNA replication and the GAS41 promoter, in chicken myelomonocytic HD11 cells arrested in G0, G0 and S phases as well as at the G0/S border. The results show that the chromatin of this region became more nuclease sensitive when cells were arrested in G0 phase and that this change in chromatin structure was reversible after the cells began to enter S phase.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Transcription Factors
/
Chromatin
/
Cell Cycle
/
DNA Replication
Type of study:
Practice guideline
Limits:
Humans
Language:
English
Journal:
Biol. Res
Journal subject:
Biology
Year:
2007
Type:
Article
/
Project document
Affiliation country:
Germany
Institution/Affiliation country:
Federal Agricultural Research Centre/DE
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