Mutaciones en genes gyrA y gyrB en cepas de bacilos Gram negativos aisladas en hospitales chilenos y su relación con la resistencia a fluoroquinolonas / Mutations in gyrA and gyrB genes among strains of Gram-negative bacilli isolated from Chilean hospitals and their relation with resistance to fluoroquinolones
Rev. méd. Chile
;
135(9): 1103-1110, sept. 2007. tab
Article
in Spanish
| LILACS
| ID: lil-468197
ABSTRACT
Background:
A progressive frequency of resistance to fluorquinolones is observed among Gram-negative bacilli.Aim:
To investigate the mechanism of resistance to fluoroquinolones mediated by mutations affecting gyrA and gyrB genes in strains of Gram negative bacüli isolated from CMean hospitals. Material andmethod:
Minimal inhibitory concentration of fluoroquinolones was determined in 91 randomly selected nalidixic acid-resistant strains of Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii and Pseudomonas aeruginosa, isolated from hospitals of 12 Chilean cities. Quinolone resistance determining region (QRDR) was amplified by PCR and mutations were determined by restriction fragment length polymorphism (RFLP) and DNA sequencing.Results:
Strains with mutation in codon 83 of gyrA showed decreased susceptibility to ciprofloxacin with MICs ranging from 0.25 to 1024 fig/ml. The sequencing of PCR products for gyrA indicated amino acid changes in the QRDR region. One strain ofE. coli presented a double mutation, in codon 83 Ser to Leu as well as in codon 87 Asp to Asn. In strains ofK. pneumoniae, however, the change of codon 83 was Ser to Tyr, in A. baumannii was Ser to Leu and in P. aeruginosa was Thr to He. No strains with mutations affecting gyrB were found.Conclusions:
Mutations in codon 83 of gyrA is a frequent genetic event involved in the mechanism leading to decreased susceptibility to fluoroquinolone in strains of Gram-negative bacilli.
Full text:
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Index:
LILACS (Americas)
Main subject:
Bacterial Proteins
/
Fluoroquinolones
/
DNA Gyrase
/
Drug Resistance, Bacterial
/
Gram-Negative Bacteria
/
Anti-Bacterial Agents
/
Mutation
Limits:
Humans
Country/Region as subject:
South America
/
Chile
Language:
Spanish
Journal:
Rev. méd. Chile
Journal subject:
Medicine
Year:
2007
Type:
Article
/
Project document
Affiliation country:
Chile
Institution/Affiliation country:
Universidad San Sebastián/CL
/
Universidad de Concepción/CL
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