Susceptibility of strict and facultative anaerobes Isolated from endodontic infections to metronidazole and beta-lactams
J. appl. oral sci
;
15(6): 539-545, Nov.-Dec. 2007. graf, tab
Article
in English
| LILACS
| ID: lil-471114
ABSTRACT
Endodontic infections are mixed aerobic-anaerobic infections and several microbial groups associated to these pathologies are also involved in orofacial infections. The goal of this study was to evaluate the susceptibility of microorganisms isolated from endodontic infections to beta-lactams and metronidazole and verify the production of beta-lactamases. Clinical specimens were collected from 58 endodontic infections of 52 patients. The microorganisms were isolated in selective and non-selective culture media, under anaerobiosis and aerobiosis, and identified using biochemical methods. In the susceptibility tests, it was used an agar dilution method, and Wilkins-Chalgren agar enriched with blood, hemin and menadione for the anaerobes, while Mueller-Hinton agar was employed for the facultative anaerobes. The production of beta-lactamases was evaluated through the biological and chromogenic cephalosporin methods. All tested isolates were sensitive to imipenem and 99.3 percent to amoxicillin/clavulanate association, while 16.1 percent showed resistance to amoxicillin and penicillin G, and 4.89 percent to cefoxitin. Resistance to metronidazole was just found in facultative anaerobes. Production of beta-lactamases was detected in 18.2 percent of the isolates and presented a correlation with resistance to beta-lactams.
Full text:
Available
Index:
LILACS (Americas)
Language:
English
Journal:
J. appl. oral sci
Journal subject:
Dentistry
Year:
2007
Type:
Article
/
Project document
Affiliation country:
Brazil
Institution/Affiliation country:
School of Dentistry of Santa Fé do Sul/BR
/
São Paulo State University/BR
/
UNIC/BR
/
UNIRP/BR
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