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Construction of a recombinant plasmid pSH-G containing the rabies-virus glycoprotein G gene
Carnieli Junior, P; Ventura, A. M; Durigon, E. L.
  • Carnieli Junior, P; Pasteur Institute. São Paulo. BR
  • Ventura, A. M; University of São Paulo. Institute of Biomedical Sciences. Department of Microbiology. São Paulo. BR
  • Durigon, E. L; University of São Paulo. Institute of Biomedical Sciences. Department of Microbiology. São Paulo. BR
J. venom. anim. toxins incl. trop. dis ; 13(4): 874-880, 2007. ilus
Article in English | LILACS | ID: lil-471147
ABSTRACT
A plasmid named pSH-G was constructed with the rabies-virus G-gene insert. This plasmid was transfected into eukaryotic BHK-21 cells and its stability tested. The presence of the pSH-G plasmid was confirmed by means of polymerase chain reaction (PCR) after each of ten cell passages, and the results were positive. The stable BHK-21/pSH-G+ clone obtained can be used in the study of rabies as well as in the production of vaccines.
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Full text: Available Index: LILACS (Americas) Main subject: Plasmids / Rabies virus / Recombinant Fusion Proteins / Glycoproteins / Transfection Language: English Journal: J. venom. anim. toxins incl. trop. dis Journal subject: Toxicology Year: 2007 Type: Article Affiliation country: Brazil Institution/Affiliation country: Pasteur Institute/BR / University of São Paulo/BR

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Full text: Available Index: LILACS (Americas) Main subject: Plasmids / Rabies virus / Recombinant Fusion Proteins / Glycoproteins / Transfection Language: English Journal: J. venom. anim. toxins incl. trop. dis Journal subject: Toxicology Year: 2007 Type: Article Affiliation country: Brazil Institution/Affiliation country: Pasteur Institute/BR / University of São Paulo/BR