DNA flow cytometry of canine mammary tumors: comparative aspects with human breast tumors
Arq. bras. med. vet. zootec
;
59(5): 1163-1168, out. 2007. graf, tab
Article
in English
| LILACS
| ID: lil-471197
ABSTRACT
Flow cytometric analysis of DNA content was performed on 28 samples of canine mammary tumors. Nine of them were benign and 19 were malignant. All benign tumors and 11 malignant tumors (57.9 percent) were diploid (P<0.05). Form the aneuploid tumors, five (26.3 percent) were hyperdiploid, one (5.3 percent) hypodiploid, one (5.3 percent) near triploid and one (5.3 percent) multiploid. The analysis of the expression of the markers PR and CD31 revealed a significant difference between diploid and aneuploid tumors (P<0.05). The immunoreactivity of PR was higher in diploid tumors, while the immunoreactivity of CD31 was stronger in aneuploid tumors. No difference between the markers MIB-1, c-erbB2, p53 and Cyclin D1 was observed (P>0.05). Using the flow cytometry analysis and immunohistochemistry, it was found a close relationship between aneuploidy and malignant character of neoplasias, progesterone receptor (PR) negative immunostaining and higher microvases density. No correlation between DNA content and S phase or immunoreactivity for the markers MIB-1, p53, c-erbB2 and Cyclin D1 was observed
RESUMO
Análise por citometria de fluxo de DNA foi realizada em 28 amostras de tumores mamários de cadela. Nove eram benignos e 19 malignos, sendo todos os benignos e 11 malignos (57,9 por cento) diplóides (P<0,05). Dos tumores aneuploides, cinco (26,3 por cento) eram hiperdiploides, um (5,3 por cento) hipodiploide, um (5,3 por cento) triploide e um (5,3 por cento) multiploide. A análise dos marcadores de expressão PR e CD31 revelaram significativa diferença entre tumores diploides e aneuploides (P<0,05). A imunorreatividade do PR foi maior em tumores diplóides e a imunoreatividade do CD 31 maior em tumores aneuploides. Para os marcadores MIB-1, c-erbB-2, p53 e Ciclina D1 não foi observada diferença significativa (P>0,05). Pela citometria de fluxo e pela imunoistoquímica verificaram-se uma relação entre aneuploidia e características malignas das neoplasias, receptor de progesterona imunoreação negativa e alta densidade de microvascular. Não foi observada correlação entre conteúdo de DNA e a fase S ou imunorreatividade para os marcadores MIB-1, c-erbB-2, p53 e Ciclina D1
Full text:
Available
Index:
LILACS (Americas)
Main subject:
DNA
/
Immunohistochemistry
/
Mammary Neoplasms, Animal
/
Dogs
/
Flow Cytometry
Limits:
Animals
Language:
English
Journal:
Arq. bras. med. vet. zootec
Journal subject:
Veterinary Medicine
Year:
2007
Type:
Article
Affiliation country:
Brazil
/
Portugal
Institution/Affiliation country:
UFMG/BR
/
Universidade do Porto/PT
Similar
MEDLINE
...
LILACS
LIS